Fig. 7
The respiratory burst (ROS production) assay. Leukocytes (2.0 10^6 cells/ml) of age-matched controls (n = 12), COPD patient groups 1 (n = 17) and COPD patient group 2 (n = 19) were pre-incubated in the presence or absence of PAF (10−6 M); loaded with DHR 123 for 5 min and stimulated with fMLF (a-f) for 15 min at 37 °C. Production of ROS was measured by oxidation of intracellular DHR 123 and measured using flow cytometry (Mean ± SEM). An independent t-test (** = p < 0.01, * = p < 0.05) was used to perform statistical analysis