Fig. 5

Lateral heat-map for MCT behaviour in sheep trachea. Two dimensional heat-map showing the effect of aerosolised hypertonic on the predominant MCT particle velocity across the three relevant sheep tracheal segments (n = 2 sheep) over the 10-minute imaging period. The heat-map orientation is the same as Fig. 3b and d; the dorsal tracheal surface is to the left and the ventral tracheal surface to the right. Stationary particles are at the origin, slow moving particles are close to the axis origin, and faster moving particles are further away, with the angular position representing their direction of motion. Red indicates more particles than yellow or blue. The heat maps indicate that the bulk of the particle motion is in a cranial direction, with a bias towards the dorsal tracheal surface (the average angle of particle transport was -14.3° from vertical)