Table 1 List of primers and their corresponding sequences used for PCR analysis
From: Role of heparin in pulmonary cell populations in an in-vitro model of acute lung injury
Gene | Forward primer | Reverse primer |
|---|---|---|
GAPDH | 5′ GAT CAT GAG CAA TGC CTC CT 3′ | 5-TGT GGT CAT GAG TCG TTC CA-3 |
TNF-α | 5′TCC TTC AGA CAC CCT CAA CC 3′ | 5-AGG CCC CAG TTT GAA TTC TT-3 |
IL8 | 5′ ATTTCTGCAGCTCTGTGTGAAGGTGC 3′ | 5 -TTGTGGATCCTGGCTAGCAGA C-3 |
MCP1 | 5′ CAAACTGAAGCTCGCACTCTCGCC 3, | 5 -ATTCTTGGGTTGTGGAGTGAGTGTTCA-3 |
IRAK1 | 5′ CCAGCCCCTTCTTCTACCAA 3′ | 5′ AGCATACACCGTGTTCCTCA 3′ |
TGF-β | 5′ CGGATCAGCGTTTATCAGGT 3′ | 5′ CAACTTGGGGTTGATGCTCT3′ |
Myd88 | 5′ TCACCACACTTGATGACCCC 3′ | 5′ CGGCACCTCTTTTCGATGAG 3′ |
SMAD2 | 5′ TAAAGTGCCTGGGATTGAGG 3′ | 5′ GTGTGCCTGGGACTTGTTTT 3′ |
SMAD3 | 5′ ATAGGTGCTTTGGGCGTATG 3′ | 5′ CTGCTATCCAGTCACCAGCA 3′ |
IL6 | 5′ TACCCCCAGGAGAAGATTCC 3′ | 5′ TTTTCTGCCAGTGCCTCTTT 3′ |