Fig. 4
From: Role of heparin in pulmonary cell populations in an in-vitro model of acute lung injury
Human fibroblasts gene expression. a Expression of TNF-α, IL-6, IL-8, MCP-1, IRAK-1, MyD88, TGF-β, Smad2 and Smad3 evaluated by q-PCR at 24 h after LPS treatment. Data are expressed mean ± SEM (ΔCt correction was applied using GAPDH as a housekeeping gene and units are relative to the expression of control group) (n = 8 samples per group). b Protein expression for TNF-α, IL-6, IL-8, MCP-1 and TGF-β (n = 4 samples per group). Data are expressed mean ± SEM group c Immunofluorescence for NF-kß, IRAK-1, MyD88 and Smad2/3 and all the treatments are shown. Magnification is 400x. *p ≤ 0.05 vs control groups; #p ≤ 0.05 vs LPS group (LPS: Lipopolysaccharide from Escherichia coli 055:B5 and HEP: unfractionated heparin)