Fig. 1
From: Role of heparin in pulmonary cell populations in an in-vitro model of acute lung injury
Purity of isolated cells. hAM were stained with Diff quick to differentiate macrophages, neutrophils and lymphocytes and also CD68 immunofluorescence. hATII were stained with alkaline phosphatase and also with Surfactant C protein immunofluorescence. hF were stained with Diff Quick and ACTA2 immunofluorescence. Diff quick and alkaline phosphatase images had x400 magnification. Images of Hoechst, CD68, SPC and ACTA2 immunofluorescences had x600 magnification (hAM: human alveolar macrophages; hATII: human alveolar type II cells; hF: human fibroblasts, SPC: surfactant C protein and ACTA2: alpha smooth actin)