Fig. 6

CDHR3 is predominantly expressed by ciliated epithelial cells and diminishes following C15 inoculation. PC-differentiated cultures inoculated or mock-inoculated for 18 h with C15 (C15+) or BEGM alone (Mock), respectively, were labeled with antibodies against C15 capsid (C15), CDHR3, and aat (cilia) and analyzed by flow cytometry (n = 6). a Relative median fluorescence intensity (rMFI) of CDHR3 in ciliated (Yes) and nonciliated (No) cell populations of mock-inoculated cultures. b Frequency of CDHR3 positive ciliated cells out of the total ciliated cell population in mock- and C15-inoculated cultures. c rMFI of CDHR3 in ciliated cells negative (C15-) or positive (C15+) for C15 staining. Values are normalized to the double-negative (nonciliated and CDHR3-) population in each experiment. ** p ≤ 0.01, * p ≤ 0.05