Fig. 2

Analysis of C15 infectivity by immunofluorescent staining. HBEC-ALI cultures were infected for 18 h with C15 and imaged apically and z-stacked orthogonally by confocal microscopy. Secretory and ciliated cells were stained with WGA and rabbit polyclonal antibody against aat, respectively (false color red), C15 capsid stained with monoclonal antibody (false color green), nuclei stained with Syto-13 (false color blue). Scale bars indicate 20 μm