Fig. 1

C15 inoculation of airway epithelial cells causes a speckled pattern of infection and infected cell shedding. HBEC-ALI cultures were inoculated for 18 h with C15 or media alone and imaged by fluorescent microscopy (a and b, respectively). Nuclei stained with Hoechst (blue), C15 capsid stained with monoclonal antibody against VP2 (red). Inoculated cultures were also imaged by confocal microscopy and analyzed by z-stacking (c and d) or apical surface views (e and f). Nuclei stained with Syto-13 (green), secretory cells stained with WGA (blue), and C15 capsid stained with monoclonal antibody (orange). Scale bars indicate 20 μm