Fig. 8

Forskolin and procaterol have similar effects. a, b Human eosinophils were pre-treated with procaterol or forskolin for 30 min and then co-cultured with serum-starved BEAS-2B for 24 h before analyzing integrin expression by flow cytometry. c BEAS-2B cells were collected to evaluate the mRNA expression of E-cadherin and vimentin. d Co-culture supernatants were collected to evaluate the levels of transforming growth factor (TGF-β1) and granulocyte-macrophage colony-stimulating factor (GM-CSF). e EMT of BEAS-2B cells were evaluated in each treatment group. Bars indicate mean ± SEM. Scale bars indicate 100 μm. The data are the representative of a single experiment performed in triplicates. *p < 0.05 vs control groups; **p < 0.05 vs eosinophils co-cultured with BEAS-2B in the absence of both procaterol and forskolin. Statistics by analysis of variance with Dunnett’s test