Fig. 3
A specific β2 adrenergic receptor inhibitor blocks the effect of procaterol. a Control and BEAS-2B cells co-cultured with human eosinophils in the presence or absence of procaterol and butoxamine (original magnifications, ×400). b Gene expression of E-cadherin and vimentin as evaluated by RT-PCR. c Granulocyte-macrophage colony-stimulating factor (GM-CSF) and transforming growth factor (TGF-β1) levels in the supernatant. Bars indicate mean ± SEM. Scale bars indicate 100 μm. The data are the representative of a single experiment performed in triplicates. Three independent experiments were performed. *p < 0.005 vs butoxamine (−)/procaterol (−) group; **p < 0.005 vs butoxamine (−)/procaterol (+) group. Statistics by analysis of variance with Dunnett’s test