Fig. 2

EMT induced by primary human eosinophils is inhibited by procaterol. a Control and BEAS-2B cells co-cultured with primary human eosinophils in the presence or absence of procaterol (original magnifications, ×400). b Gene expression of E-cadherin and vimentin as assessed by RT-PCR. c Granulocyte-macrophage colony-stimulating factor (GM-CSF) and transforming growth factor (TGF-β1) levels in the supernatant. d Representative immunofluorescence staining of E-cadherin (green) and α-SMA (red) in BEAS-2B cell with saline or eosinophils or eosinophils pre-treated with procaterol. e Quantification by densitometry. Bars indicate mean ± SEM. Scale bars indicate 100 μm. The data are the representative of a single experiment performed in triplicates. Two independent experiments were performed. *p < 0.001 vs procaterol (−) group; **p < 0.001 and ¶p < 0.05 vs eosinophils (+)/procaterol (−) group. Statistics by analysis of variance with Dunnett’s test