Skip to main content
Fig. 5 | Respiratory Research

Fig. 5

From: Secretion of the endoplasmic reticulum stress protein, GRP78, into the BALF is increased in cigarette smokers

Fig. 5

a and b Brefeldin A (BF-A) inhibits fibronectin (FN) without affecting GRP78 secretion by HAEC. Cells were pre-treated for 3.5 h with BF-A (0, 5, 15 or 25 μg/ml) then treated for 24 h with TG (1 μM) + HDAC inhibitors (vorinostat + MS275 + trichostatin A, all 2 μM) or vehicle (DMSO 0.2% and ethanol 0.1%). a Representative Western blot showing that BF-A dose-dependently inhibited fibronectin but not GRP78 secretion. One experiment of 2. b Group mean data ± SEM of 2 experiments showing ratio of medium GRP78/cell lysate GRP78 (closed symbols) and medium FN/cell lysate FN (open symbols). Values were normalized to vehicle control. Error bars for FN are smaller than the symbols. The decrease in FN ratio was significant (p = 0.03 by ANOVA)

Back to article page

Respiratory Research

ISSN: 1465-993X

Contact us