Fig. 4

Transduction efficiency by a single endobronchial administration of Ad-lacZ as evaluated by ex vivo endobronchial staining with lacZ assays. a Ad-lacZ was delivered into the left lungs by a single endobronchial administration. Fourteen days later, the lungs were stained for 4 h with intratracheal administration of 0.5 mg/ml X-gal (13). Left: Control, Right: Ad-lacZ. b, c The lung tissues were sectioned into 5-μm slices, and counter-stained with nuclear fast red (Kernechtrot). Magnification × 200, Bar = 40 μm. b: Control, c: Ad-lacZ