Table 1 Outlining surface marker antigens and relevant conjugated monoclonal antibodies used to identify various cell populations
From: Characterisation of a murine model of the late asthmatic response
Cell Type | Surface marker Phenotype | Monoclonal Antibody Conjugate |
|---|---|---|
B-cells | CD19+ | Rat anti-mouse CD19 (FITC) |
CD4+ T-cells | CD4+ | CD4 (PerCP) |
CD8+ T-cells | CD8+ | CD8 (APC) |
Neutrophils | Ly-6Ghigh CD11bhigh CD11clow F4/80low | Ly6G (FITC) CD11b (PerCP) CD11c (APC) F4/80 (PE) |
Eosinophils | CD11bhigh CD11clow Ly6Glow SiglecFhigh | CD11b (PerCP) CD11c (APC) Ly6G (FITC) SiglecF (PE) |
Alveolar Macrophages | CD11blow-intermediate CD11chigh F4/80high Ly-6Glow | CD11b (PerCP) CD11c (APC) F4/80 (PE) Ly6G (FITC) |
Inflammatory monocytes/tissue macrophages | CD11bhigh CD11clow F4/80high | CD11b (PerCP) CD11c (APC) F4/80 (PE) |