Fig. 6

AMPK-dependent mTOR signaling pathways are distinctly dysregulated in RV of MCT rat model. a Western blot analysis of total and phosphorylated levels of AMPK, mTOR, and p70S6 kinase in RV protein extracts from control and MCT treated rats for 2, 4, and 6 weeks. β-actin was used as a loading control. b-d Quantification of phosphorylated levels of AMPK, mTOR, and p70S6K to total AMPK, mTOR and p70S6K ratios. e-g Quantification of AMPK, mTOR, and p70S6 protein band signal densities was performed using ImageJ and plotted normalized to β-actin levels. Values are presented as mean ± standard deviation. ^* P < 0.05, ^** P < 0.01