Fig. 3

Histological alterations in the RV during compensatory hypertrophy and heart failure in the MCT-treated rats. a-d Cardiomyocyte cross-sectional area and myocardial interstitial fibrosis [using wheat-germ-agglutinin staining (WGA) and Masson trichrome staining, respectively] from control and MCT rat model. Representative images of WGA stained cardiomyocytes from control and MCT treated rats at 2, 4, and 6 weeks. White scale represents 20 μm (×400 original magnification); (e-h) Representative images of Masson trichrome-stained RV tissues from control and MCT treated rats at 2, 4, and 6 weeks. Red stain denotes muscle fibers. Blue stain denotes collagen. Black scale represents 50 μm (×200 original magnification); (i) Quantitative analysis of cardiomyocyte cross-sectional (transverse) area; (j) Quantitative analysis of interstitial fibrotic area. ^* P < 0.05, ^** P < 0.01. Values are presented as mean ± standard deviation