Fig. 2
Evaluation of intrapulmonary arterial contraction in pulmonary arterial hypertension models. Contractions of rat intrapulmonary arterial rings induced by cumulative concentrations of phenylephrine (PHE, 10−11 to 10−5M, a), or of prostaglandin F2α (PGF2α, 10−9 to 10−4M, b). Contractions were studied ex vivo after sacrifice at Day 28 in control rats (CTRL), after chronic hypoxia (Hx), after monocrotaline treatment (MCT), of after combination of MCT and 4 weeks of Hx (MCT + Hx). Results are expressed as the percentage of maximal contraction and are presented as means ± SEM with n = 12–15 rats per group. Determination of statistically significant differences between concentration-response curves was assessed with a two-way analysis of variance. ***p < 0.001 versus control concentration-response curves. ### p < 0.001 versus Hx concentration-response curves. $$$ p < 0.001 versus MCT concentration-response curves. Values of -Log EC50 (half maximal effective concentrations, means ± SEM) are shown in Table c