Fig. 6

Alterations in REDD1 but not Akt and mTOR phosphorylation levels in response to pulmonary inflammation. Mice were intratracheally instilled with elastase and LPS or respective vc. Protein levels of (b) phosphorylated mTOR (ser2448), (c) total mTOR, (f) phosphorylated Akt (ser473) and (g) total Akt were assessed in lysates of gastrocnemius muscle tissue with western blot analysis. d Representative western blots of indicated proteins. Ratio of (a) phosphorylated mTOR over total mTOR and (e) phosphorylated Akt over total Akt. h mRNA transcript levels of REDD1 were determined, normalized to geNorm, and expressed as fold change compared to control. n = 8–12 per group. * p < 0.05, ** p < 0.01, *** p < 0.001, # represents a trend