Fig. 5
From: Pleural inhibition of the caspase-1/IL-1β pathway diminishes profibrotic lung toxicity of bleomycin
Caspase-1 inhibition hampers pleural structural cell transformation. a, b Met5A cells were cultured with a BLM or b TGF alone or in combination with caspase-1 inhibitor YVAD (western blot; left, densitometric analysis; right). Expression of α-SMA and E-Cadherin were assessed by western blot. HSC70 and β-actin: loading control. Representative results from three independent experiments. c, d qPCR analysis of E-Cadherin and α-SMA mRNA on Met5A cells cultured in the presence of nigericin (0.1 μM) or control c at the indicated times or d for 24 h. C57Bl/6 mice were given intrapleural injections of nigericin (1.25 mg/kg) or control. e Histological analysis at D21 post-intrapleural injection. Representative observations after H&E staining, scale bars: 100 μm. f Total cell count in the PLF at D14 after intrapleural injection of nigericin or control. *p ≤ 0.05, **p ≤ 0.01