Fig. 2From: Proteases and oxidant stress control organic dust induction of inflammatory gene expression in lung epithelial cellsEffects of protease inhibitors on inflammatory gene induction. A549 (a), Beas2B (b) and SAE (c) cells were treated with medium, dust extract (DE) (0.25 %) alone or dust extract (0.25 %) in the presence of 25 μg/ml α1-antitrypsin (α1-AT) or 25 μg/ml soybean trypsin inhibitor for 3 h. Beas2B cells (d) were first treated with medium or medium containing metalloproteinase inhibitor GM6001 (10 μM) for 1 h and then exposed to medium or dust extract (DE) (0.25 %) for 3 h. Levels of mRNAs were determined by qRT-PCR. Levels of each mRNA in dust extract treated cells were arbitrarily considered as 100 and relative levels in cells treated with a combination of dust extract and inhibitors are shown. Data are means ± SE (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant, compared with cells treated only with dust extractBack to article page