Fig. 6
From: Vimentin dephosphorylation at ser-56 is regulated by type 1 protein phosphatase in smooth muscle
PP1 regulates myosin light chain phosphorylation at Ser-19 in smooth muscle. Mouse tracheal rings treated with control (Ctrl) or PP1 shRNA were stimulated with 10−4 M ACh for 5 min, or left unstimulated. Myosin light chain (MLC) phosphorylation at Ser-19 in the tissues was evaluated by immunoblot analysis. MLC phosphorylation under various treatments is normalized to the phosphorylation in unstimulated tissues treated with control shRNA. Data are mean ± SE (n = 4). *, p < 0.05