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Fig. 5 | Respiratory Research

Fig. 5

From: Cigarette smoke alters the ability of human dendritic cells to promote anti-Streptococcus pneumoniae Th17 response

Fig. 5

In vitro exposure to cigarette smoke extract (CSE) is associated with higher endocytosis of S. pneumoniae (Sp) by monocyte-derived dendritic cells (MDDC). a Mean fluorescence intensity (MFI) of internalized dye-labelled Sp was measured by flow cytometry. One condition was put at 4 °C to inhibit endocytosis as a negative control. b Proportion of viable internalized Sp after one hour exposure were quantify to measure bacteria endocytosis as described by Zhou [20]. c After one more hour incubation, internalized still viable Sp were quantify to measure the proportion of killed Sp. Data represent mean ± S.E.M. of 4 and 2 experiments for a and b-c respectively. There was no statistical difference between groups

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Respiratory Research

ISSN: 1465-993X

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