Fig. 9
From: Ajulemic acid exerts potent anti-fibrotic effect during the fibrogenic phase of bleomycin lung
AjA treatment in the fibrogenic phase significantly decreases the expression of α-SMA in lungs of BLM treated-mice. a Immunohistochemical staining of α-SMA at day 21 after BLM delivery in lung section from control mouse, BLM-treated mouse with consolidated area of fibrosis, and mice receiving at day 8 from BLM delivery AjA at different doses. Scale bars = 60 μm. b Immunohistochemical score of α-SMA in the lungs of the different experimental groups. * p < 0.05 vs control; † p < 0.05 vs BLM. c Representative Western blot analysis of α-SMA from total lung samples of control, BLM-instilled and AjA-treated mice at 21 days after BLM challenge. Lane (1) = control sample. Lane (2) = BLM + AjA 5 mg. Lanes (3) = BLM + AjA 1 mg. Lanes (4) = BLM-instilled sample