Fig. 5
From: Ajulemic acid exerts potent anti-fibrotic effect during the fibrogenic phase of bleomycin lung
AjA treatment is followed by a reduced expression of α-SMA. Representative immunohistochemical staining of α-SMA in lung sections from animals from the different experimental groups at day 14 a and 21 b after BLM treatment, receiving AjA at 1 h before BLM challenge. Scale bars = 60 μm. c Immunohistochemical score for α-SMA in the different experimental groups. Data are reported as mean ± SD. *p < 0.05 vs control group. † p < 0.05 vs BLM group. d Representative Western blot analysis of α-SMA from total lung samples of control, BLM-instilled and AjA-treated mice at 14 and 21 days after the start of treatments. Lanes (1) and (3) = Control samples. Lanes (2) and (4) = BLM-instilled mice at 14 and 21 days. Lanes (5) and (7) = AjA 1 mg at 14 and 21 days. Lanes (6) and (8) = AjA 5 mg at 14 and 21 days