Fig. 4
Colocalization of Cav-1 and tight junction proteins in an in vitro pulmonary epithelial barrier model. Alveolar cell monolayers were exposed to normoxia (N) (21 % O2/5 % CO2) or hyperoxia (H) (85 % O2/5 % CO2) for 72 h. The colocalization of Cav-1 and tight junction proteins in the pulmonary epithelium in vitro was monitored by double immunofluorescence staining. a–d, green represents ZO-1 and occludin, respectively, and red represents Cav-1. e and f, green represents Cav-1 and red represents claudin-4. Yellow represents the colocalization of Cav-1 and tight junction proteins (800× magnification)