Fig. 2

TGF-β1 induces WNT10A expression in fibroblasts. a The results of an analysis of Hoechst (blue), WNT10A (red) and Phalloidin (green) staining in LL97A cells as indicated by immunofluorescence. The WNT10A expression after the administration of TGF-β1 (5 ng/ml) in LL97A cells was analyzed using a Western blot analysis. b LL97A and IMR-90 cells were treated with TGF-β1 (5 ng/ml), and at the indicated time points, the mRNA level of WNT10A was assessed by real-time qPCR compared to the untreated cells, and the results are expressed as the means ± SEM. ^* p < 0.05. c The WNT family genes (WNT3A, WNT5A, WNT7B, WNT10A, WNT10B and β-catenin) were analyzed by real-time qPCR compared to the untreated cells, and the results are expressed as the means ± SEM. ^* p < 0.05. d The luciferase activity of the WNT10A promoter was measured 24 h after TGF-β1 (5 ng/ml) stimulation, and the results are expressed as the means ± SEM. ^* p < 0.05