Fig. 4

The effects of IL-25 and IL-33 on markers of basophil activation. Purified peripheral blood basophils from 8 allergic donors were incubated for 18 h with or without IL-25 or IL-33 (both 10 ng/mL) and neutralizing antibodies for IL-17RB and ST2 or isotype controls (both 10 μg/mL). Receptor blockade (light grey bars) was assessed by comparison to isotype controls (black bars) via measuring: (a) CD203c, (b) IL-3Rα, (c) intracellular IL-13, and (d) intracellular IL-4. Anti-IgE and IL-3 (10 ng/mL) served as positive controls (dark grey bars). Data are expressed as mean ± SEM. *Significantly different from PBS negative control, † significantly different from isotype control P < 0.05 (N = 8)