Fig. 5

Effect of BMAL1 on TGF-β1-induced EMT of HPL1D lung epithelial cells. HPL1D cells were transiently transfected with siBMAL1 or siCtrl for 48 h. During the last 24 h of transfection, the cells were treated with TGF-β1 at 5 ng/ml. Real-time qRT-PCR analysis was employed to investigate the expression of BMAL1 (a), E-cad (b) and FN-EDA (c). d HPL1D cells were transiently transfected with siBMAL1 or siCtrl for 72 h. During the last 48 h of transfection, the cells were treated with TGF-β1 at 5 ng/ml. Western blot analysis was carried out to investigate the E-cad and FN-EDA expression. e & f Densitometry analysis of Western blots in (d). * p < 0.05 vs. corresponding control siRNA groups stimulated with TGF-β1 (siCtrl-TGF-β1)