Fig. 5

Blocking of EGFR reduces the stimulatory effect of MSC-CM^STIM on wound closure. a: NCI-H292 wounded cell layers were stimulated with MSC-CM^STIM or DMEM^STIM, DMEM^CTRL, or DMEM supplemented with TGF-α 20 ng/ml or HGF 20 ng/ml (positive controls). Inhibitors of EGFR (0.2 μM AG1478) or c-Met (0.05 μM PF04217903) were added during the full culture period in assigned conditions. At 48 h, HGF and TGF-α both significantly enhanced wound healing in NCI-H292 cells, which was averted to a level comparable to the negative control by their respective inhibitors. b: In the presence of the EGFR inhibitor, the wound healing capacity of MSC-CM^STIM decreased to values below those observed in the negative control as determined after 48 h. Inhibition of c-Met alone had no effect on wound healing induced by MSC-CM^STIM. Box and whiskers represent median, interquartile range and minimum and maximum. n = 4-7; (*) p < 0.05