Fig. 2
MSC-CM increases wound closure. a, b: NCI-H292 cells were injured by making a circular wound with a diameter of 3 mm, and subsequently incubated with MSC-CMCTRL, MSC-CMSTIM, DMEMCTRL and DMEMSTIM, and a negative control (NC, RPMI only) and positive control (RPMI supplemented with TGF-α 20 ng/ml). The wound size was measured at 0, 24, 48 and 72 h after wounding. MSC-CMSTIM significantly increased wound closure. The effect of MSC-CMSTIM was dose-dependent. Error bars represent standard error of the mean (SEM). n = 4-6; (*) p < 0.05 TGF-α compared to NC and (**) compared to DMEM; (***) p < 0.05 MSC-CM compared to NC and (****) compared to DMEM; (*****) p < 0.05 DMEMSTIM compared to NC. c: Morphology of the closure of the wound: photos in the upper panel are taken at t = 0 h, the lower panel shows the same wounds photographed 48 h later. The two photos on the left side are obtained from MSC-CMSTIM stimulated cells, whereas photos at the right represent its control, DMEMSTIM. d: Dose response. Box and whiskers represent median, interquartile range and minimum and maximum. n = 4-6; (*) p < 0.05. e: Wound closure in ALI-PBEC measured at 6 and 24 h (at 48 h all wounds were closed). At 24 h, MSC-CMSTIM significantly enhanced wound healing compared to the NC, but no significant differences were observed compared to its control, DMEMSTIM. Error bars represent SEM, n = 4; (*) p < 0.05 for MSC-CMSTIM compared to NC. f. Wound closure in ALI-PBEC at 24 h. Box and whiskers represent median, interquartile range and minimum and maximum, for n = 4 as in Fig. 2f; (*) p < 0.05