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Fig. 3 | Respiratory Research

Fig. 3

From: Activation of Src-dependent Smad3 signaling mediates the neutrophilic inflammation and oxidative stress in hyperoxia-augmented ventilator-induced lung injury

Fig. 3

SIS3 and Src-deficient mice attenuated hyperoxia-augmented high-tidal-volume-induced MMP-9 activation, MMP-9 mRNA expression, and MMP-9 production. a Gelatin zymography of MMP-9 activity and b real-time polymerase chain reaction of MMP-9 mRNA expression were from the lungs of nonventilated control mice and those subjected to a tidal volume of 30 mL/kg for 1 h with room air or hyperoxia. Arbitrary units were the results of MMP-9 gelatinase activity normalized to the nonventilated control wild-type mice with room air and the ratio of MMP-9 mRNA to GAPDH (n = 5 per group). c MMP-9 protein production in BAL fluid were from the lungs of nonventilated control mice and those subjected to a tidal volume of 30 mL/kg for 4 h with room air or hyperoxia (n = 5 per group). SIS3 2-mg/kg was administered intraperitoneally 1 h before ventilation. *P < 0.05 versus the nonventilated control mice with room air; † P < 0.05 versus all other groups. MMP-9 = matrix metalloproteinase-9

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Respiratory Research

ISSN: 1465-993X

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