Fig. 6

Role of NADPH oxidase in septic PMVEC permeability barrier dysfunction, death and apoptosis 4 h following CLP-sepsis in mice in vivo. As compared to septic WT mice, NADPH oxidase deficiency (both gp91 ^phox−/− and p47 ^phox−/−) or inhibition (apocynin) was associated with complete attenuation of septic increases in (a) pulmonary microvascular EB-albumin leak, (b) PMVEC death, and (c) PMVEC apoptosis (TUNEL+). n = 3–4 (Sham) and n = 3–6 (CLP). * p < 0.05 and ** p<0.01, CLP vs. respective sham group; #, p < 0.05 vs. wild-type CLP