Fig. 9

Localization of phospho-Stat3 and expression of IL-6 at early fibrotic stage of BLM-injured lung. Lung sections from mice instilled with BLM (8 dpi) were used for the following experiments. a Masson’s trichrome staining. b Lung sections were stained with anti-IL-6 and anti-proSP-C antibodies followed by reaction with Alexa 488- or Alexa 594-conjugated second antibodies (488 for IL-6; 594 for SP-C). Arrowhead indicates IL-6⁺SP-C⁺ cell. c Lung sections were stained with anti-IL-6 and anti-Iba1 antibodies followed by reaction with Alexa 488- or Alexa 594-conjugated second antibodies (488 for IL-6; 594 for Iba1). Arrowheads indicate IL-6⁺Iba1⁺ cells. d Lung sections were stained with anti-IL-6 and anti-S100A4 antibodies followed by reaction with Alexa 488- or Alexa 594-conjugated second antibodies (488 for IL-6; 594 for S100A4). e Lung sections were stained with anti-proSP-C and anti-phospho-Stat3 antibodies followed by reaction with Alexa 488- or Alexa 594-conjugated second antibodies (488 for SP-C; 594 for phospho-Stat3). f Lung sections were stained with anti-SMA and anti-phospho-Stat3 antibodies followed by reaction with Alexa 488- or Alexa 594-conjugated second antibodies (488 for SMA; 594 for phospho-Stat3). Nuclei were stained with DAPI. Similar results to the histopathological changes and immunofluorescence profiles were observed in four independent experiments