Fig. 1

Effect of BLM on phosphorylation of signaling proteins in lung. Lysates (400 μg) from the lungs of mice instilled with PBS or BLM (3 dpi) were subjected to Pathscan Antibody Array. a Typical profile of BLM-induced protein phosphorylation. Proteins with increased phosphorylation levels in response to BLM are boxed. In particular, phospho-Akt and -Stat3 are boxed by a double-line. b Phosphorylation levels of these proteins in the lungs of mice instilled with PBS (white bars) and BLM (black bars). Using a densitometer, each signal was normalized to the positive internal controls included in the array glass and expressed in arbitrary units. Average signal of the positive internal controls is expressed as 10 arbitrary units. Data are shown as mean ± S.E.M. of three independent experiments. The difference between the two groups (PBS and BLM) was statistically significant (*P < 0.05) by Student’s t-test for unpaired values