Fig. 3

Allele-specific quantitation in M/S and M/Z heterozygotes. a. Total and wild-type A1AT were quantitated in M/S heterozygotes (n = 63) by LC-MS/MS. Total A1AT concentration determined from the proteotypic peptide (black bars) was compared to the wild-type M concentration as measured by the non-S peptide (gray bars). b. Total and wild-type A1AT were quantitated in M/Z heterozygotes (n = 61) by LC-MS/MS. Total A1AT concentration determined from the proteotypic peptide (black bars) was compared to the wild-type M concentration as measured by the non-Z peptide (gray bars). c. The concentration of non-S (M protein) and non-Z (total) in each M/S sample was expressed as percent of total A1AT concentration as measured by the proteotypic peptide (average ± 1 standard deviation are shown). d. The concentration of non-Z (M protein) and non-S (total) in each M/Z sample was expressed as percent of total A1AT concentration as measured by the proteotypic peptide (average ± 1 standard deviation are shown)