Fig. 8

scFv A1 does not initiate or interfere with Kit signalling. HMC-1 were incubated with scFv A1 or E4 control in the presence or absence of SCF 100 ng/ml. Total intracellular phosphorylation assessed by Western blotting (PY99 antibody) was not altered by A1 in either the absence or presence of SCF. Phosphorylation of Kit (bands 145/120 kDa) in the left panel was also not altered. Staining for Kit (E1, right panel) and β-actin demonstrates equal loading for Kit within each pair of experiments. Representative of three independent experiments