Fig. 7

scFv1 A1 binds to human Kit-expressing MCBS1 mouse mast cells. Kit expression was confirmed by flow cytometry (a) and immunofluorescence (b). a Fluorescent histograms (red lines), plotted with corresponding isotype controls (blue line) demonstrate A1 and Kit expression in the W1-AA677 human Kit-expressing mouse mast cell line, but not in E1-AA685 mock-transfected cells or non-transfected control cells. The mean fluorescent intensity was determined minus the total binding of matched isotype controls for A1 and Kit, scFv E4 and mouse IgG respectively. Representative of 3 independent experiments. b Immunofluorescent staining of W1-AA677, E1-AA685 and control cells. Nuclei stained blue with DAPI, A1 and Kit stained red (RPE); inset: isotype control. Representative of two independent experiments