Fig. 4

The effects of scFv A1 on mast cell adhesion. a HMC-1 adhesion to monolayers of BEAS-2B human airway epithelial cells and primary HASMCs was assessed in the presence of anti-mast cell scFv A1 or a control scFv E4 which does not bind. Baseline adhesion and adhesion in control media containing antibody vehicle (glycerol, final concentration 0.8-1.5 % depending on the scFv batch) is also shown. Plotted as mean ± SEM for all experiments; n = 8 ASM donors used in independent experiments and n = 4 independent experiments for BEAS-2B. *p = 0.048 compared to E4, **p = 0.008 compared to E4. b HLMC adhesion to monolayers of BEAS-2B human airway epithelial cells and primary HASMCs was assessed in the presence of anti-mast cell scFv A1 or a control scFv E4 which does not bind. Baseline adhesion and adhesion in control media containing antibody vehicle (glycerol, final concentration 0.8-1.5 % depending on the scFv batch) is also shown for HASMCs, baseline adhesion is shown for BEAS-2B cells (glycerol not assessed). Plotted as mean ± SEM for all experiments; n = 1 ASM donor and 7 HLMC donors used in n = 7 independent experiments, and n = 6 HLMC donors in independent experiments for BEAS-2B. *p = 0.029 compared to E4, # p = 0.017 compared to E4