Fig. 6
From: Increased FGF1-FGFRc expression in idiopathic pulmonary fibrosis
Impact of exogenous FGF1 on cell death and cell proliferation on fibroblasts from IPF and donor lungs. FACS plots (a) represent flow cytometry performed on treated donor and IPF fibroblasts for Annexin V (detected with the FL4A channel) and propidium iodide (detected with the FL2A channel). Cells were starved for 24 h and then treated once daily for two days with heparin (25 ng/mL) recombinant human FGF1 (25 ng/mL), FGF1 + heparin together, FGF1 + heparin + PD173074 (0.1 μM resuspended in DMSO), or as positive control 1uM Staurosporine (resuspended in DMSO) 18 h before harvest for experiment. Graphic representation of the percentage of apoptotic cells (Annexin V positive and propidium iodide negative) for donor fibroblasts (20 % vs. 40 %) (b) and IPF fibroblasts (18 % vs. 50 %) (b’); n = 4 biological samples/treatment group. The gating strategy is shown in Additional file 6: Figure S5. Western blot for proliferating cell nuclear antigen (PCNA) against GAPDH (c). Densitometry plots of arbitrary units indicated no significant regulation of PCNA in various treatment groups (d,d’)