Table 1 Real-time PCR primers for genes
From: Hyperoxia-induced methylation decreases RUNX3 in a newborn rat model of bronchopulmonary dysplasia
Target gene | 5′ → 3′ | Product | |
|---|---|---|---|
dnmt1 | Sense | CATGGTGCTGAAGCTCACAC | 176Â bp |
Antisense | GGGCAAACACGTGTAGAGGT | ||
dnmt3b | Sense | TTCTCATGATGCCAAAGCTC | 118BP |
Antisense | GAGGTTCTTTGCCTCTCCAG | ||
ezh2 | Sense | TTCGTTTTGCTAATCATTCAGTAA | 162Â bp |
Antisense | CCACATACTTCAGGGCATCA | ||
runx3 | Sense | GAAGATAGAGGACCAGACCAAAG | 194Â bp |
Antisense | GGAAGGAGCGATCAAACTG | ||
gapdh | Sense | AGACAGCCGCATCTTCTTGT | 207Â bp |
Antisense | CTTGCCGTGGGTAGAGTCAT | ||