Fig. 7
3D matrices induce Tenascin-C synthesis in lung fibroblasts. Levels of Tenascin-C (TNC) were measured from the supernatants of the 3D DMEM matrices by ELISA. The results were obtained from three independent experiments. Fibroblasts were cultured with 10 % of FBS. The synthesis of TNC was induced in a time-dependent manner, observing significant statistical differences between the 1st and the 14th and 21st days of culture (p < 0.05 for both comparisons). Although no differences were appreciated between the 14th and the 21st days of culture, a higher level of TNC was observed with 30 mM of ribose at the 21st day (p = 0.08). No statistical differences were observed between glycated and non-glycated matrices. These results suggest that the 3D microenvironment could be enough to produce changes in the secretion of TNC by normal fibroblasts in a time-dependent manner