Table 1 Primers for quantitative real-time RT-PCR analyses of gene transcript expression
Gene | Forward primer | Reverse primer | Size (bp) |
|---|---|---|---|
E-cadherin | 5’-gccgagagctacacgttca-3’ | 5’-gaccggtgcaatcttcaaa-3’ | 88 |
N-cadherin | 5’-ctccatgtgccggatagc-3’ | 5’-cgatttcaccagaagcctctac-3’ | 92 |
Vimentin | 5’-gtttcccctaaaccgctagg-3’ | 5’-agcgagagtggcagagga-3’ | 68 |
Snail | 5’-gctgcaggactctaatccaga-3’ | 5‘-atctccggaggtgggatg-3’ | 84 |
Slug | 5’-tggttgcttcaaggacacat-3’ | 5‘-gttgcagtgagggcaagaa-3’ | 66 |
Twist1 | 5’-aaggcatcactatggactttctct-3’ | 5‘-gccagtttgatcccagtatttt-3’ | 96 |
Twist2 | 5’-tctgaaacctgaacaacctcag-3’ | 5’-ctgctgtcccttctctcgac | 70 |
Zeb1 | 5’-gctaagaactgctgggaggat-3’ | 5’-atcctgcttcatctgcctga-3’ | 79-82 |
Zeb2 | 5’-aagccagggacagatcagc-3’ | 5’-ccacactctgtgcatttgaact-3’ | 74 |
HGF | 5’-cagagggacaaaggaaaagaa-3’ | 5’-gcaagtgaatggaagtccttta-3’ | 167 |
GAPDH | 5’-agtcaacggatttggtcgtatt-3’ | 5’-atgggtggaatcatattggaac-3’ | 139 |