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Table 1 Primers for quantitative real-time RT-PCR analyses of gene transcript expression

From: Fibroblast-epithelial cell interactions drive epithelial-mesenchymal transition differently in cells from normal and COPD patients

Gene Forward primer Reverse primer Size (bp)
E-cadherin 5’-gccgagagctacacgttca-3’ 5’-gaccggtgcaatcttcaaa-3’ 88
N-cadherin 5’-ctccatgtgccggatagc-3’ 5’-cgatttcaccagaagcctctac-3’ 92
Vimentin 5’-gtttcccctaaaccgctagg-3’ 5’-agcgagagtggcagagga-3’ 68
Snail 5’-gctgcaggactctaatccaga-3’ 5‘-atctccggaggtgggatg-3’ 84
Slug 5’-tggttgcttcaaggacacat-3’ 5‘-gttgcagtgagggcaagaa-3’ 66
Twist1 5’-aaggcatcactatggactttctct-3’ 5‘-gccagtttgatcccagtatttt-3’ 96
Twist2 5’-tctgaaacctgaacaacctcag-3’ 5’-ctgctgtcccttctctcgac 70
Zeb1 5’-gctaagaactgctgggaggat-3’ 5’-atcctgcttcatctgcctga-3’ 79-82
Zeb2 5’-aagccagggacagatcagc-3’ 5’-ccacactctgtgcatttgaact-3’ 74
HGF 5’-cagagggacaaaggaaaagaa-3’ 5’-gcaagtgaatggaagtccttta-3’ 167
GAPDH 5’-agtcaacggatttggtcgtatt-3’ 5’-atgggtggaatcatattggaac-3’ 139