Fig. 5

The regulation role of p120 on the RhoA activation induced tight junction dysfunction. MLE-12 cells were transfected with p120 siRNA, p120 cDNA or pretreated with Y27632 before 20 % cyclic stretches. (a, b) The level of occludin and p120 were detected by western blot in p120 siRNA or cDNA transfected group after cyclic stretch. (c) The effects of cyclic stretch on the association of occludin and ZO-1. (d, e) The association of occludin and ZO-1 was detected by immunoprecipitation in p120 siRNA or cDNA transfected group after cyclic stretch. (f, g) The activity of RhoA was examined by pull down assay in p120 siRNA group or p120 cDNA group. *p < 0.05 versus control group, +p < 0.05 versus CS group. Experiments were repeated at least three times