Table 1 Primer sequences for real-time PCR
From: TWEAK enhances TGF-β-induced epithelial-mesenchymal transition in human bronchial epithelial cells
Target | Sense primer(5′→3′) | Antisense primer(5′→3′) |
|---|---|---|
E-cadherin | ACCTCCATCACAGAGGTTCC | GAAGCCGAGGTTTTAACTGC |
Zo-1 | GAGGACCAGCTGAAGGACAG | TGCATTTTTCCCACTTTTCC |
N-cadherin | AGCCAACCTTAACTGAGGAGT | GGCAAGTTGATTGGAGGGATG |
Vimentin | AATTGCAGGAGGAGATGCTT | GAGACGCATTGTCAACATCC |
Tenascin-C | CTGGACTTGCTCCCAGCATC | CCAGGAAACTGTGAACCCGTA |
HAS-2 | GTGATGACAGGCATCTCA | GCGGGAAGTAAACTCGA |
Fibronectin | GAAGCCGAGGTTTTAACTGC | ACCCACTCGGTAAGTGTTCC |
Snail | CTCTTTCCTCGTCAGGAAGC | GGCTGCTGGAAGGTAAACTC |
Slug | CCAAACTACAGCGAACTGGA | GTGGTATGACAGGCATGGAG |
Twist | CCGGAGACCTAGATGTCATTG | CCACGCCCTGTTTCTTTG |
ZEB1 | AACCCAACTTGAACGTCACA | ATTACACCCAGACTGCGTCA |
ZEB2 | CAGACCGCAATTAACAATGG | TACTCCTCGATGCTGACTGC |
GAPDH | GGTCTCCTCTGACTTCAACA | GTGAGGGTCTCTCTCTTCCT |