Figure 4

3D depiction of principal component analysis (PCA) of gene expression in cultures treated with vehicle, 30 μM CdCl ₂ , or 100 μM CdCl ₂ . Sixty five genes that met the criteria defined by the RT² Profiler PCR Array Data Analysis v3.5 software from SABiosciences were uploaded to the ArrayTrack program. The PCA is based on the log₂ ratios and expression profiles across all the 65 genes in the PCR array. The blue, green, and red dots indicate cultures treated with vehicle, 30 μM CdCl₂, and 100 μM CdCl₂, respectively. The first three principal components are plotted. The captured variance of PC1 (first principal component), PC2 (second principal component; the label is not shown), and PC3 (third principal component) were 45.79%, 31.70%, and 10.12%, respectively.