Figure 5

AAL-R increases lymphocyte apoptosis/necrosis in the lungs. Single-cell suspensions were obtained from the lungs to determine: A) the frequency of apoptotic/necrotic annexin V⁺ cells in two subpopulations of cells, namely AF^neg SSC^low CD90.2⁺ CD8^neg CD4⁺ T cells (upper panels) and AF^neg SSC^low CD90.2^neg CD19⁺ B cells (lower panels); B) the number of CD4⁺ T cells; and C) the number of CD19⁺ B cells in the lung. Total number of eosinophils was also determined in the BALF (D). Shown is representative of two independent experiments. n = 6–8 mice per group and *indicates significant differences versus VEH; ^§indicates significant differences versus AAL-R. P <0.05.