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Figure 4 | Respiratory Research

Figure 4

From: Expression of surfactant protein D in airways of asthmatics and interleukin-13 modulation of surfactant protein D in human models of airway epithelium

Figure 4

Surfactant protein D (SP-D) expression in non-asthmatic ALI cultures treated with IL-13. SP-D expression was examined in IL-13-treated (10 ng/ml, 14-day duration) ALI sections grown from matched non-asthmatic donors via immunohistochemistry, where pink was indicative of positivity [A]. Fraction of total epithelial area positive was quantified using colour segmentation in ImagePro Plus. Expression of SP-D in the airway epithelia decreased significantly in the cultures treated with IL-13 (n = 4, t-test p = 0.0005) [B]. SP-D protein expression in ALI cultures treated with IL-13 for 24 hours, 7 days, and 14 days were detected by Western blotting, quantified by densitometry, and normalized to untreated control to obtain fold changes. All three durations resulted in reductions of SP-D expression (n = 6, one-way ANOVA p = 0.02) [C].

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Respiratory Research

ISSN: 1465-993X

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