IL-27 regulates cytokine production during influenza infection. C57BL/6, WT and IL-27Rα−/− mice, were infected with 100 PFU of influenza A PR/8/34 for 7 days. Type 17-associated inflammatory cytokine mRNA gene expression in lung homogenate was measured by RT-PCR, n = 7 (A) and cytokine/chemokine levels were measured in lung homogenate by Lincoplex, n = 7 (B-F). IL-10 mRNA gene expression in lung homogenate was measured by RT-PCR, n = 7 (G). Viral burden was determined by measuring influenza matrix protein mRNA gene expression in lung homogenate by RT-PCR, n = 7 (H). *p < 0.05 IL-27Rα−/− versus WT mice. All data are reported as means ± SEM for each group by unpaired t test. Data are the combined results of two replicate experiments.