Figure 7
From: Regulation of IL-17A responses in human airway smooth muscle cells by Oncostatin M
Effects of STAT3 pharmacologic inhibition on HASMC responses. HASMC cultures were prepared and stimulated, as previous, with the indicated cytokines IL-4, IL-13, or IL-17A at 5 ng/ml in the absence or presence of 1 ng/ml OSM. Parallel cultures were stimulated in the presence of either (A) 2.5 uM Stattic (black bars), (B) 10 uM SB203580 (dashed lines) or (C) 5 uM Akt X (white bars). Cell lines were stimulated for 18 hours and cell culture supernatants were collected and assessed by ELISA for levels of MCP-1/CCL-2 (left panel) and IL-6 (right panels). *p < 0.05; **p < 0.01, ***p < 0.001 comparing presence and absence of the indicated inhibitor in each cytokine treatment with 1 ng/ml OSM using Two-Way ANOVA with Bonferroni’s post-test.