Figure 1

RAGE TG mice fed doxycycline (dox) up-regulated RAGE in CCSP-expressing cells of the proximal airways. Dox administration commenced on post natal (PN) 20 and continued until sacrifice date on PN 60. Quantitative RT-PCR (A) and immunoblotting (B) for RAGE revealed significant increases in RAGE expression following dox treatment of RAGE TG animals compared to dox-fed controls. RAGE detection using qPCR and blotting was conducted using samples with equal mRNA or protein concentrations from each animal as described. Data are representative of at least 4 animals per group and *p ≤0.05. RAGE immunohistochemistry revealed normal distal lung expression in both groups (C and D, arrowheads). No RAGE expression was detected in the airways of control mice (C, arrow); however, punctate RAGE expression was common in the airways of RAGE TG mice (D, arrows). Representative images (400× original magnification) of n =3 mice in each group are shown.